We have formulated a simple roadmap whereby the necessary gene systems maybe developed and deployed. At its heart is the use of 'pseudo suicide' vectors and the creation of a pyrE mutant (a uracil auxotroph), initially aided by ClosTron technology, but ultimately made using a special form of allelic exchange termed ACE (Allele Coupled Exchange). All mutants, regardless of the mutagen employed, are made in this host. Purpose With the incredible economy growth of China, the number of the China's… Continue